Detectable Pathogens
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Avian Aspergillosis
Avian aspergillosis is caused by a filamentous fungus, Aspergillus fumigatus, but there are others such as A. flavus, A. niger, A. and A. nidulans terreus can also produce (1.5). The spores of these fungi are widely distributed in the environment and many birds can carry up to stimuli such as immunosuppression or stress triggers the onset of clinical symptoms such as loss of appetite, lethargy, loss weight, dyspnea, sometimes diarrhea and abnormal behavior and posture. The disease is usually a respiratory tract infection, although it can enter the blood (3,5).
Because the symptoms of the disease depend on several factors, clinical signs alone do not have a reliable basis for the diagnosis of avian aspergillosis, which is further complicated by the variety of pathogens associated with (2.5). Bioingentech can detect avian aspergillosis using the following techniques (1):
Technique |
Reference |
Conventional polymerase chain reaction |
Einsele et al., 2003(4) |
Real Time polymerase chain reaction |
Katoh et al., 2008(2) |
Bibliography
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Abdin MZ, Ahmad MM, Javed S. Advances in molecular detection of Aspergillus: an update. Arch Microbiol. 2010 Jun;192(6):409-25.
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Bolehovska R, Pliskova L, Buchta V, Cerman J, Hamal P. Detection of Aspergillus spp. in biological samples by real-time PCR. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2006 Nov;150(2):245-8.
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Cacciuttolo E, Rossi G, Nardoni S, Legrottaglie R, Mani P. Anatomopathological aspects of avian aspergillosis. Vet Res Commun. 2009 Feb 3.
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Einsele H, Hebart H, Roller G, Löffler J, Rothenhofer I, Müller CA, Bowden RA, van Burik J, Engelhard D, Kanz L, Schumacher U. Detection and identification of fungal pathogens in blood by using molecular probes. J Clin Microbiol. 1997 Jun;35(6):1353-60.
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Krishnan S, Manavathu EK, Chandrasekar PH. Aspergillus flavus: an emerging non-fumigatus Aspergillus species of significance. Mycoses. 2009 May;52(3):206-22.
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Logotheti M, Kotsovili-Tseleni A, Arsenis G, Legakis NI. Multiplex PCR for the discrimination of A. fumigatus, A. flavus, A. niger and A. terreus. J Microbiol Methods. 2009 Feb;76(2):209-11.
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