Newcastle Disease

There are nine known serotypes of avian paramyxovirus (PMV-1 to PMV-9). The virus of Newcastle disease (EN) is the PMV-1 and is the most important pathogen of the group to birds, but the PMV 2, 3, 6 and 7 may occasionally sick chickens and turkeys.
One of the most characteristic properties of different strains of virus EN, is the enormous variation in the pathogenicity for chickens. Virus strains of EN are grouped into five pathotypes based on clinical signs observed in infected chickens.
As the signs of clinical disease in chickens vary widely, and the diagnosis may be complicated further by the different responses to infection in different hosts, clinical signs alone do not have a reliable basis for the diagnosis of EN. However, clinical signs and lesions associated with virulent pathotypes will provide a strong suspicion of disease. (1, 2)
Definitive diagnosis of EN, it is necessary to isolate the virus and molecular characterization of the subtypes. Bioingentech can detect the virus of Newcastle disease using the following techniques, supported by the World Organisation for Animal Health (OIE) (2, 3):

Bibliography

• Alexander DJ. Newcastle disease and other avian paramyxoviruses. Rev Sci Tech. 2000 Aug;19(2):443-62.
• OIE, Código Sanitario para los Animales Terrestres 2010, vols. I & II, 19a ed., 2010.
• Sambrook J, Frisch EF, Maniatis T. Molecular Cloning, A laboratory manual, 2a ed. 1990.